mouse bone marrow mesenchymal stem cells  (ATCC)

Journal: Journal of Nanobiotechnology

Article Title: Supramolecular delivery of senolytics enables targeted anti-senescence therapy and accelerated fracture healing

doi: 10.1186/s12951-026-04138-2

Figure Lengend Snippet: Asp 10 SAC4A enables hypoxia-activated senolysis and promotes osteogenic differentiation in vitro. A ) Heatmap of combination index (CI) values at different D: Q ratios and fraction affected (Fa) levels, showing synergistic effects of Dasatinib and Quercetin (DQ). B ) Cell viability curves of normal BMSCs, senescent BMSCs (Sn-BMSCs), and Sn-BMSCs under hypoxia treated with vehicle, free DQ, Asp 10 SAC4A, or DQ@Asp 10 SAC4A. C ) Representative SA-β-Gal staining images of senescent cells treated with different formulations under normoxic and hypoxic conditions, and quantification of SA-β-Gal-positive area (%) ( n = 4/group). Scale bar: 100 μm. D–F ) Representative Western blot images ( D ) and quantitative analyses ( E , F ) of senescence markers P16 and P21 expression in different treatment groups ( n = 3/group). G ) Representative alkaline phosphatase (ALP, upper panel) and Alizarin Red staining (ARS, lower panel) images demonstrating osteogenic differentiation after indicated treatments under normoxic and hypoxic conditions. H–J ) Representative Western blot images ( H ) and quantitative analyses ( I , J ) showing protein expression levels of osteogenic markers RUNX2 and osteopontin (OPN) ( n = 3/group). (Data are presented as mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001; n = 3–4/group)

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from Servicebio (Catalog number STCC6011P).

Techniques: In Vitro, Staining, Western Blot, Expressing

Journal: Bioactive Materials

Article Title: Biomimetic bone niche reconstructs proliferation-inhibited and therapy-resistant bone-metastatic prostate cancer

doi: 10.1016/j.bioactmat.2025.09.041

Figure Lengend Snippet: Single-cell data revealed a subset of proliferation-arrested cells in bone metastatic prostate cancer. a) Classification of tumor cells into three subpopulations based on anatomical origin: distal site, involved site, metastatic tumor site. b) UMAP projection of single-cell transcriptomes from spinal metastatic tissues of 9 prostate cancer patients, annotated by cell type. c) Heatmap displaying DEGs across the three tumor cell subpopulations. d) Heatmap of differentially enriched Hallmark pathways (GSEA) among the tumor subpopulations. e) Schematic overview of the analysis pipeline for primary tumor cells from 11 patients who underwent radical prostatectomy. f) UMAP projection of single-cell transcriptomes from radical prostatectomy specimens, annotated by identified cell types. g) UMAP plot of epithelial cells from radical prostatectomy tissues showing 17 clusters (0–16); clusters 1 and 11 identified as malignant via inferCNV analysis. h) Schematic summarizing the integrated analysis of primary and metastatic tumor cell datasets. i) Volcano plot of DEGs between primary and distal tumor cells. j) Comparison of Hallmark pathway enrichment related to cell cycle and proliferation between primary and distal tumor cells (GSEA). k) Expression levels of representative genes associated with mitosis and prostate cancer proliferation in primary versus distal tumor cells. l) UMAP projection of integrated single-cell data from distal tumor cells, MSCs, and osteoblasts. m) Volcano plot of signature genes distinguishing distal tumor cells, MSCs, and osteoblasts. n) Ligand-receptor interaction network among distal tumor cells, MSCs, and osteoblasts, identified using the iTALK algorithm. o) Top 20 ligand-receptor interactions between MSCs/osteoblasts and tumor cells predicted by iTALK.

Article Snippet: Cell lines and culture: Primary mouse bone marrow mesenchymal stem cells (mBMSCs) were obtained from Procell Bioscience Inc. (China).

Techniques: Comparison, Expressing

Journal: Bioactive Materials

Article Title: Biomimetic bone niche reconstructs proliferation-inhibited and therapy-resistant bone-metastatic prostate cancer

doi: 10.1016/j.bioactmat.2025.09.041

Figure Lengend Snippet: Biocompatibility and osteoinductive properties of the engineered BME. a) Schematic and representative fluorescence images showing individual culture of MC3T3 (mCherry), MSC (GFP), and C4-2B (BFP) cells in dECM hydrogels. Representative x-y plane projections at days 1, 3, 7, 14, and 21. b) Cell proliferation of MC3T3, MSC, and C4-2B in dECM hydrogels over 21 days (n = 3). c) Schematic and fluorescence images showing MC3T3 attachment to the CPC scaffold and subsequent co-culture with MSCs. d) Proliferation dynamics of MC3T3 and MSCs in the hydrogel-based BME model over 14 days (n = 3), quantified as fluorescence intensity normalized to Day 1. e) ALP staining at day 7. f) ARS at day 14. g) Expression of osteogenic genes at day 7. h) Images of the BME model 7 days after implantation and upon retrieval at day 28. i, j, k) Histological and immunohistochemistry analysis of retrieved tissues: H&E staining (i), Masson trichrome staining (j), and immunostaining of osteogenic markers (k). Statistical analysis was performed using one-way ANOVA (n = 3). Significance is indicated as: ns (not significant), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, respectively.

Article Snippet: Cell lines and culture: Primary mouse bone marrow mesenchymal stem cells (mBMSCs) were obtained from Procell Bioscience Inc. (China).

Techniques: Fluorescence, Co-Culture Assay, Staining, Expressing, Immunohistochemistry, Immunostaining

Journal: Bioactive Materials

Article Title: Biomimetic bone niche reconstructs proliferation-inhibited and therapy-resistant bone-metastatic prostate cancer

doi: 10.1016/j.bioactmat.2025.09.041

Figure Lengend Snippet: Cell interactions and drug resistance in the 3D-printed BME . a) Schematic illustrating cellular interactions among prostate cancer cells, MSCs, and osteoblasts within the BME. b) Ligand-receptor interaction network among cancer cells, MSCs, and osteoblasts in the BME, identified using the iTALK algorithm. c) Top 20 ligand-receptor pairs between MSCs/osteoblasts and tumor cells in the BME (iTALK analysis). d, e) Comparison of the top 100 ligand-receptor pairs from bulk RNA-seq and single-cell RNA-seq data, highlighting overlapping interactions between MSCs/osteoblasts and tumor cells. f) Schematic and fluorescence images showing tumor spheroids cultured in BME or control (3D-only) conditions under 10 μM enzalutamide treatment; images captured on days 0, 3, 5, and 7. g) Following enzalutamide administration, a quantitative comparison of the relative proliferation (normalized to Day 1) of C4-2B tumor spheroids between the control and BME-treated groups was conducted over time (Days 1, 3, 5, and 7). h) Schematic of flow cytometry sorting and analysis of dissociated tumor cells from BME and control groups, stained for live/dead and apoptosis markers. i, j) Flow cytometry quantification of dead and apoptotic tumor cells in BME and control groups on days 1 and 7 post-enzalutamide treatment. k) KEGG enrichment analysis of DEGs showing altered metabolism-related pathways in BME compared to control. l) GSEA highlighting differences in MYC targets (V1) and fatty acid metabolism pathways between BME and control tumor cells. m) Kaplan-Meier curve showing association of BME-derived gene signatures (bulk RNA-seq) with reduced progression-free survival in TCGA prostate cancer cohort (p = 0.0576). n) Kaplan-Meier curve showing association of distal tumor gene signatures (single-cell RNA-seq) with reduced progression-free survival in TCGA dataset (p = 0.0595). A paired t -test was employed to ascertain significance: ns (not significant), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, respectively.

Article Snippet: Cell lines and culture: Primary mouse bone marrow mesenchymal stem cells (mBMSCs) were obtained from Procell Bioscience Inc. (China).

Techniques: Comparison, RNA Sequencing, Fluorescence, Cell Culture, Control, Flow Cytometry, Staining, Derivative Assay

Journal: The Journal of International Medical Research

Article Title: Preparation and properties of polyvinyl alcohol/calcium phosphate composite bone cement

doi: 10.1177/03000605251389734

Figure Lengend Snippet: Cell morphology and growth of each group after 72 h of culture. (a) α-mBMSCs cultured in normal culture with complete medium. (b) mBMSCs co-cultured with 5% PVA/CPC composite. (c) mBMSCs cultured with 5% PVA/CPC composite extract. α-mBMSCs: alpha-mouse bone marrow mesenchymal stem cells; CPC: calcium phosphate cement PVA: polyvinyl alcohol.

Article Snippet: Mouse bone marrow mesenchymal stem cells (mBMSCs) were provided by Wuhan Procell Life Science & Technology Co., Ltd.

Techniques: Cell Culture

Journal: The Journal of International Medical Research

Article Title: Preparation and properties of polyvinyl alcohol/calcium phosphate composite bone cement

doi: 10.1177/03000605251389734

Figure Lengend Snippet: The effect of PVA fibers on the migration ability of mBMSCs was determined using the Transwell method (n ≥ 6). PVA: polyvinyl alcohol; mBMSCs: mouse bone marrow mesenchymal stem cells.

Article Snippet: Mouse bone marrow mesenchymal stem cells (mBMSCs) were provided by Wuhan Procell Life Science & Technology Co., Ltd.

Techniques: Migration